Vol. 2 No. 3 (2025)

Research Articles

The Effect of HPV Mixed Infection and HPV Single Infection on Vaginal Microecology and Their Correlation Analysis

Published 2025-07-30

  • Na Tang
    • ,
  • Yan Zhang

Na Tang
Laboratory Department of Xiangya Boai Rehabilitation Hospital (Fuyuan District), Changsha 410100, China

Yan Zhang
Laboratory Department of Xiangya Boai Rehabilitation Hospital (Fuyuan District), Changsha 410100, China

Abstract

Human papillomavirus (HPV) is a major pathogen factor for cervicitis and cervical cancer, it mediates vaginal microecology changes to cause diseases occurrence. But its mechanism is full clear. This study investigated the effect of human papillomavirus (HPV) infection on vaginal microecology and analyzed its correlation. 300 female patients who visited in Xiangya Boai Rehabilitation Hospital from January 2023 to December 2023 were enrolled into this study. HPV of the patients was detected using PCR method. The patients were diagnosed by two clinical experts. According to the HPV infection, the patients were divided into HPV single model infection group and HPV mixed group, with 100 cases in each group. 100 cases of the healthy population without HPV infection and cervicitis served as normal control group. Subsequently, HPV and vaginal microecological detections were performed on all subjects, including vaginal secretion cleanliness, PH value, leukocyte esterase (LE), sialic acid (SNA), hydrogen peroxide (H2O2), and N-acetyl-β-galactosaminase (NAG). According to the test statistics, among the patients with HPV infection, the patients with HPV 51, 52, 53, 58 accounted for the majority. Compared with the normal control group and the HPV single infection group, the HPV mixed infection group had statistically significant differences in vaginal cleanliness, PH, positive rate of hydrogen oxidase, epithelial cells, LE, SNA, H2O2 and NAG in vaginal microecology (P < 0.01). In general, HPV mixed infection has a significant effect on vaginal microecology, while HPV single infection has little effect on vaginal microecology. These results can provide reference for clinical prevention and treatment of cervical lesions.

Keywords

Human papillomavirus (HPV), Leukocyte esterase (LE), Sialidase (SNA), Hydrogen peroxide (H2O2), N-acetyl-β-galactosaminase (NAG), Vaginal microecology

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